Project
EWOBS

Part:BBa_J45800:Design

Designed by: MIT iGEM 2006   Group: iGEM06_MIT   (2006-10-31)


Exponential phase wintergreen odor biosynthetic system


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3620
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 389
    Illegal NgoMIV site found at 793
    Illegal NgoMIV site found at 918
    Illegal NgoMIV site found at 929
    Illegal NgoMIV site found at 1210
    Illegal NgoMIV site found at 1678
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3161


Design Notes

We recommend use of strain BBa_J45999 as it does not produce indole. Indole is responsible for the foul smell of wt e.coli, and masks the wintergreen scent produced by J45800.



Source

This part is composed of BBa_J45320 and BBa_J45180

References